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Accession Number PB2013-106377
Title NIJ Proposal to Enhance Methods for Studying Degraded DNA, Final Technical Report.
Publication Date Feb 2013
Media Count 165p
Personal Author B. M. Kemp C. Grier C. Monroe J. E. Teisberg J. L. Barta K. Flanigan M. Winters
Abstract Quantitative PCR was used to evaluate the efficacy of sodium hypochlorite in the removal of contaminating DNA from bone surfaces. While our findings are consistent with previous studies that found sodium hypochlorite to be highly efficient ((approximately)81-99%) at contamination removal, there emerged no treatment that removed 100% of the contamination across all of the experiments. Furthermore, this study suggests that previous claims that sodium hypochlorite is particularly damaging to endogenous ancient DNA (aDNA) molecules are inaccurate. Experiments conducted during this phase of the grant led to two additional relevant observations. First, mitochondrial DNA (mtDNA) preservation across individual bones was determined to be highly variable and not related to the density of the bone material, despite previous belief of such a relationship. Secondly, utilizing qPCR and a synthesized standards approach to measure the efficiency of some common DNA extraction methods for degraded skeletal samples, all methods were determined to perform poorly in retaining short segments of DNA. These findings challenge low copy number (LCN) expectations, suggesting that ancient and forensic specimens may contain far more preserved genetic material than previously recognized.
Keywords Bone surfaces
Contamination removal
DNA
Genetic species
Molecules
Sodium hypochlorite

 
Source Agency Department of Justice General
NTIS Subject Category 57F - Cytology, Genetics, & Molecular Biology
57B - Biochemistry
Corporate Author National Criminal Justice Reference Service, Washington, DC.
Document Type Technical report
Title Note N/A
NTIS Issue Number 1316
Contract Number N/A

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