Accession Number PB2013-106277
Title Automated Processing of Sexual Assault Cases Using Selective Degradation. Final Tehcnical Report.
Publication Date Apr 2012
Media Count 75p
Personal Author A. Garvin C. Carson K. Gorman
Abstract This research addressed the problem of obtaining a sperm DNA profile from sexual assault evidence samples that also contain a large amount of epithelial cell DNA, and the ability to automate the differential extraction process. The current standard differential extraction method leaves some amount of epithelial cell DNA in the sperm fraction of the sample, which will create a mixture of epithelial and sperm cell DNA and may partially or completely mask the sperm DNA profile. This may prevent conviction or even detection of the perpetrator. This research used a nuclease to digest the remaining epithelial DNA in the sperm fraction of mock sexual assault samples. The research demonstrated the ability to obtain clean single-source male DNA profiles from mixed stains, under a variety of sample conditions. In addition, the research optimized this procedure, including reagent concentrations and volumes, and then validated the procedure for sensitivity, reproducibility and precision. Following that, the results of this selective degradation approach were compared to the results obtained with the current standard method of differential extraction. The selective degradation methodology was demonstrated to be compatible with the DNA purification methods currently used in public crime laboratories.
Keywords Automated processing
Crime laboratories
Degradation
Epithelial cell DNA
Sample conditions
Sexual assaults
Sperm


 
Source Agency Department of Justice General
NTIS Subject Category 57U - Public Health & Industrial Medicine
91C - Fire Services, Law Enforcement, & Criminal Justice
92C - Social Concerns
Corporate Author Department of Justice, Washington, DC.
Document Type Technical report
Title Note N/A
NTIS Issue Number 1314
Contract Number N/A

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