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Accession Number PB2013-100406
Title Taq Mutants Engineered for Forensics..
Publication Date Apr 2011
Media Count 44p
Personal Author M. Kermekehiev
Abstract Develop and optimize a protocol without the DNA extraction steps for direct PCR-based typing of the human STR loci from crude samples containing blood and soil using our novel OmniTaq and Omni Klentaq enzymes. Two. Develop specific PCR enhancers to improve the detection sensitivity of crude samples. Three. Test the resistance of OmniTaq and Omni Klentaq to PCR inhibitors derived from substances other than blood or soil, such as urine, semen, hair/melanin, tannins, indigo dye, bones, muscle tissue, saliva, and feces/bile salts, and extend the application of the mutant enzymes to testing crude samples of these substances. Four. Formulate and optimize blends of OmniTaq and Omni Klentaq with some members of the Y-family thermophilic polymerases with improved performance on damaged DNA.
Keywords Blood
Deoxyribonucleic acid (DNA)
Detection
Enzymes
Extraction
Forensic analysis
Genetics
Mutants
Polymerases
Sampling
Sensitivity
Soils
Testing


 
Source Agency Department of Justice General
NTIS Subject Category 57F - Cytology, Genetics, & Molecular Biology
57B - Biochemistry
Corporate Author DNA Polymerase Technology, Inc. Saint Louis, MO.
Document Type Technical report
Title Note N/A
NTIS Issue Number 1302
Contract Number 2008-DN-RX-K299

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