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Accession Number DE2013-1048513
Title Plasminogen Activator Inhibitor 1: Mechanisms of Its Synergistic Regulation by Growth Factors.
Publication Date 2011
Media Count 193p
Personal Author X. Song
Abstract My research is on the synergistic regulation of PAI-1 by EGF and TGF-beta. The mechanism of synergistic regulation of PAI-1 by EGF and TGF-beta are addressed. Methods are described for effective identification of RNA accessible sites for antisense oligodexoxynucleotides (ODNs) and siRNA. In this study effective AS-ODN sequences for both Lcn2 and Bcl2 were identified by in vitro tiled microarray studies. Our results suggest that hybridization of ODN arrays to a target mRNA under physiological conditions might be used as a rapid and reliable in vitro method to accurately identify targets on mRNA molecules for effective antisense and potential siRNA activity in vivo.
Keywords Growth factors
Hybridization
In vitro analysis
In vivo analysis
Molecular biology
Nucleotides
Physiology(Growth)
Plasminogen
Plasminogen activator
Riobonucleic acid(RNA)
Synergism
Targets


 
Source Agency Technical Information Center Oak Ridge Tennessee
NTIS Subject Category 57B - Biochemistry
57F - Cytology, Genetics, & Molecular Biology
Corporate Author Iowa State Coll., Ames.
Document Type Technical report
Title Note N/A
NTIS Issue Number 1314
Contract Number DE-AC02-07CH11358

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