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Accession Number DE2012-1052840
Title Three-Dimensional Imaging and Quantification of Biomass and Biofilms in Porous Media.
Publication Date Oct 2012
Media Count 4p
Personal Author D. Wildenschild
Abstract A new method to resolve biofilms in three dimensions in porous media using high-resolution synchrotron-based x-ray computed microtomography (CMT) has been developed. Imaging biofilms in porous media without disturbing the natural spatial arrangement of the porous media and associated biofilm has been a challenging task, primarily because porous media generally precludes conventional imaging via optical microscopy; x-ray tomography offers a potential alternative. One challenge for using this method is that most conventional x-ray contrast agents are water-soluble and easily diffuse into biofilms. To overcome this problem, silver-coated microspheres were added to the fluid phase to create an x-ray contrast that does not diffuse into the biofilm mass. Using this approach, biofilm imaging in porous media was accomplished with sufficient contrast to differentiate between the biomass- and fluid-filled pore spaces. The method was validated by using a two-dimensional micro-model flow cell where both light microscopy and CMT imaging were used to image the biofilm.
Keywords Microbial growth
Microbial plugging
Permeability reduction
Porous media
Sweep efficiency

Source Agency Technical Information Center Oak Ridge Tennessee
NTIS Subject Category 57K - Microbiology
97K - Fuels
Corporate Author Oregon State Univ., Corvallis.
Document Type Technical report
Title Note N/A
NTIS Issue Number 1307
Contract Number DE-SC0000587

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