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Accession Number
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DE2012-1052840
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Title
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Three-Dimensional Imaging and Quantification of Biomass and Biofilms in Porous Media.
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Publication Date
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Oct 2012
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Media Count
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4p
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Personal Author
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D. Wildenschild
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Abstract
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A new method to resolve biofilms in three dimensions in porous media using high-resolution synchrotron-based x-ray computed microtomography (CMT) has been developed. Imaging biofilms in porous media without disturbing the natural spatial arrangement of the porous media and associated biofilm has been a challenging task, primarily because porous media generally precludes conventional imaging via optical microscopy; x-ray tomography offers a potential alternative. One challenge for using this method is that most conventional x-ray contrast agents are water-soluble and easily diffuse into biofilms. To overcome this problem, silver-coated microspheres were added to the fluid phase to create an x-ray contrast that does not diffuse into the biofilm mass. Using this approach, biofilm imaging in porous media was accomplished with sufficient contrast to differentiate between the biomass- and fluid-filled pore spaces. The method was validated by using a two-dimensional micro-model flow cell where both light microscopy and CMT imaging were used to image the biofilm.
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Keywords
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Microbial growth
Microbial plugging
Permeability reduction
Porous media
Sweep efficiency
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Source Agency
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Technical Information Center Oak Ridge Tennessee
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NTIS Subject Category
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57K - Microbiology
97K - Fuels
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Corporate Author
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Oregon State Univ., Corvallis.
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Document Type
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Technical report
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Title Note
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N/A
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NTIS Issue Number
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1307
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Contract Number
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DE-SC0000587
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