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Accession Number DE2012-1048286
Title Molecular Analysis of the Metabolic Rates of Discrete Subsurface Populations of Sulfate Reducers.
Publication Date Apr 2011
Media Count 34p
Personal Author A. L. N'Guessan D. R. Lovey K. H. Williams M. Miletto
Abstract Elucidating the in situ metabolic activity of phylogenetically diverse populations of sulfate-reducing microorganisms that populate anoxic sedimentary environments is key to understanding subsurface ecology. Previous pure culture studies have demonstrated that transcript abundance of dissimilatory (bi)sulfite reductase genes is correlated with the sulfate reducing activity of individual cells. To evaluate whether expression of these genes was diagnostic for subsurface communities, dissimilatory (bi)sulfite reductase gene transcript abundance in phylogenetically distinct sulfate-reducing populations was quantified during a field experiment in which acetate was added to uranium-contaminated groundwater. Analysis of dsrAB sequences prior to the addition of acetate indicated that Desulfobacteraceae, Desulfobulbaceae, and Syntrophaceae-related sulfate reducers were the most abundant. Quantifying dsrB transcripts of the individual populations suggested that Desulfobacteraceae initially had higher dsrB transcripts per cell than Desulfobulbaceae or Syntrophaceae populations, and that the activity of Desulfobacteraceae increased further when the metabolism of dissimilatory metal reducers competing for the added acetate declined. In contrast, dsrB transcript abundance in Desulfobulbaceae and Syntrophaceae remained relatively constant, suggesting a lack of stimulation by added acetate. The indication of higher sulfate-reducing activity in the Desulfobacteraceae was consistent with the finding that Desulfobacteraceae became the predominant component of the sulfate-reducing community. Discontinuing acetate additions resulted in a decline in dsrB transcript abundance in the Desulfobacteraceae. These results suggest that monitoring transcripts of dissimilatory (bi)sulfite reductase genes in distinct populations of sulfate reducers can provide insight into the relative rates of metabolism of different components of the sulfate-reducing community and their ability to respond to environmental perturbations.
Keywords Abundance
Acetates
Bioremediation
Communities
Ecology
Environmental impact
Genes
Metabolism
Microorganisms
Molecular biology
Monitoring
Oxidoreductases
Population
Simulation
Sulfate

 
Source Agency Technical Information Center Oak Ridge Tennessee
NTIS Subject Category 57F - Cytology, Genetics, & Molecular Biology
68 - Environmental Pollution & Control
Corporate Author Massachusetts Univ., Amherst.
Document Type Technical report
Title Note N/A
NTIS Issue Number 1304
Contract Number DE-AC02-05CH11231

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