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Accession Number ADA583363
Title Role of Protein Kinase C Epsilon in Prostate Cancer and Metastasis.
Publication Date Aug 2013
Media Count 27p
Personal Author A. G. Uzquiza
Abstract The altered balance in the expression of PKC isozymes is a distinguished feature of cancer. One of the most notable alterations in epithelial cancers is the upregulation of PKC . This kinase has emerged as a potential oncogene and tumor biomarker, however, little is known regarding a potential causality between its upregulation and cancer development. In this research we wished to understand the role played by PKC in prostate cancer cells and establishes the proof of principle of PKC inhibition as a putative therapeutic strategy. We already found that PKC inhibition decreases size of tumor generated by PC3-ML cells in athymic/balb-c mice. Therefore, during this second year we have focus in the study of the metastasic properties of PKCe. Using an RNAi approach we found that PKC depletion markedly impaired the ability of PC3-ML metastasize in bone marrow of athymic/balb-c mice. In addition we found that this kinase is relevant in attachment, MMPs activity, cytokines expression, and anchorage-dependent and anchorage- independent growth of PC3-ML cells. Moreover, we have determined that pharmacological inhibition of PKCe significantly reduced invasiveness phenotype of PC3-ML. In summary, our results argue for a role of PKC in prostate cancer development and metastasis, highlighting its potential as a therapeutic target.
Keywords Abnormalities
Cancer development
Central nervous system
Clinical medicine
In vitro analysis
In vivo analysis
Molecular properties
Peripheral nervous system
Peripheral neuropathy
Phosphorus transferases
Prostate cancer
Signs and symptoms
Test and evaluation

Source Agency Non Paid ADAS
NTIS Subject Category 57B - Biochemistry
57E - Clinical Medicine
Corporate Author Pennsylvania Univ., Philadelphia.
Document Type Technical report
Title Note Annual summary rept. 1 Jun 2011-31 Jul 2013.
NTIS Issue Number 1401
Contract Number W81XWH-11-1-0441

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