Documents in the NTIS Technical Reports collection are the results of federally funded research. They are directly submitted to or collected by NTIS from Federal agencies for permanent accessibility to industry, academia and the public.  Before purchasing from NTIS, you may want to check for free access from (1) the issuing organization's website; (2) the U.S. Government Printing Office's Federal Digital System website; (3) the federal government Internet portal; or (4) a web search conducted using a commercial search engine such as
Accession Number ADA583112
Title Tertiary Oximes on Brain Acetylcholinesterase and Central Excitatory Effects of Nerve Agents.
Publication Date 2012
Media Count 18p
Personal Author K. A. Jeffrey M. H. John S. Tsung-Ming
Abstract Abstract. Organophosphorus nerve agents irreversibly inhibit the enzyme acetylcholinesterase (AChE), which leads to an excess of the cholinergic neurotransmitter acetylcholine in the synapses causing numerous toxic effects, including prolonged seizures and subsequent neuropathology. Current nerve agent therapies include pralidoxime (2-PAM) to reactivate inhibited AChE. The quaternary structure of this oxime does not allow it to cross the blood brain barrier (BBB) to reactivate brain AChE and to mitigate CNS toxicity. This study examined whether monoisonitrosoacetone (MINA) and N,N-diethyl-3-(2- (hydroxyimino)acetoxy) propan-1-aminium chloride (DHAP), two tertiary oximes that can penetrate the BBB, could prevent or reverse the central toxic effects of three nerve agents, sarin (GB), cyclosarin (GF), or VX, in guinea pigs. The first experiment tested whether MINA and DHAP could reactivate brain and peripheral tissue AChE inhibited by these nerve agents. Animals were challenged with a 1.0 x LD50 subcutaneous dose of a nerve agent and followed 15 min by one of 5 test doses of the oxime.
Keywords Acetylcholine
Blood brain barrier
Central nervous system
Chemical agents
Cholinergic nerves
Convulsive disorders
Gb agent
Nerve agents
Organic phosphorus compounds
Subcutaneous tissue

Source Agency Non Paid ADAS
NTIS Subject Category 74D - Chemical, Biological, & Radiological Warfare
57E - Clinical Medicine
Corporate Author Army Medical Research Inst. of Chemical Defense, Aberdeen Proving Ground, MD.
Document Type Journal article
Title Note Journal article.
NTIS Issue Number 1401
Contract Number N/A

Science and Technology Highlights

See a sampling of the latest scientific, technical and engineering information from NTIS in the NTIS Technical Reports Newsletter

Acrobat Reader Mobile    Acrobat Reader