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Accession Number ADA582839
Title Construction and Nonclinical Testing of a Puumala Virus Synthetic M Gene-Based DNA Vaccine.
Publication Date Dec 2012
Media Count 11p
Personal Author C. S. Schmaljohn J. W. Hooper M. J. Josleyn R. L. Brocato V. Wahl-Jensen
Abstract Puumala virus (PUUV) is a causative agent of hemorrhagic fever with renal syndrome (HFRS). Although PUUV-associated HFRS does not result in high case-fatality rates, the social and economic impact is considerable. There is no licensed vaccine or specific therapeutic to prevent or treat HFRS. Here we report the synthesis of a codon-optimized, full-lengthMsegment open reading frame and its cloning into a DNA vaccine vector to produce the plasmid pWRG/PUU- M(s2). pWRG/PUU-M(s2) delivered by gene gun produced high-titer neutralizing antibodies in hamsters and nonhuman primates. Vaccination with pWRG/ PUU-M(s2) protected hamsters against infection with PUUV but not against infection by related HFRS-associated hantaviruses. Unexpectedly, vaccination protected hamsters in a lethal disease model of Andes virus (ANDV) in the absence of ANDV crossneutralizing antibodies. This is the first evidence that an experimental DNA vaccine for HFRS can provide protection in a hantavirus lethal disease model.
Keywords Clones
Deoxyribonucleic acids
Fevers
Genes
Hemorrhagic fevers
Hfrs(Hemorrhagic fever with renal syndromes)
Immunization
Infectious diseases
Kidneys
Puumala virus
Renal syndrome
Signs and symptoms
Synthesis
Vaccines
Vector analysis
Viruses

 
Source Agency Non Paid ADAS
NTIS Subject Category 57E - Clinical Medicine
57J - Immunology
Corporate Author Army Medical Research Inst. of Infectious Diseases, Fort Detrick, MD. Virology Div.
Document Type Journal article
Title Note Journal article.
NTIS Issue Number 1401
Contract Number HHSN272200200016I

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