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Accession Number ADA575951
Title Developing a Drosophila Model of Schwannomatosis.
Publication Date Feb 2013
Media Count 76p
Personal Author J. A. Walker
Abstract This project examined mutations found in familial cases of schwannomatosis in the tumor suppressor SMARCB1, a component of the SWI/SNF chromatin remodeling complex. Drosophila is was used to investigate the nature of the SMARCB1 mutations and the molecular consequences which give rise to schwannomas. Sensitized in vivo assays for SMARCB1 function using RNAi knockdown of Snr1 (fly ortholog of SMARCB1) revealed missense mutations from patients have varying degrees of residual function suggesting they are hypomorphic. Drosophila genetics identified novel Snr1 interactions, including a potent interaction between Snr1 and the NF2/merlin tumor suppressor gene, which is also involved in schwannomatosis, as well as regulators of Cyclin E and the Hedgehog pathway. The consequences of SMARCB1/Snr1 knockdown on gene expression was examined using microarrays. Purification of wild type and mutant SMARCB1 was performed to examine altered protein interactions that may contribute to schwannomatosis.
Keywords Chromatin
Chromatin remodeling
Genetic interactions
In vivo analysis

Source Agency Non Paid ADAS
NTIS Subject Category 57F - Cytology, Genetics, & Molecular Biology
57E - Clinical Medicine
57K - Microbiology
Corporate Author Massachusetts General Hospital, Boston.
Document Type Technical report
Title Note Final rept. 15 Jul 2009-14 Jan 2013.
NTIS Issue Number 1319
Contract Number W81XWH-09-1-0487

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