Documents in the NTIS Technical Reports collection are the results of federally funded research. They are directly submitted to or collected by NTIS from Federal agencies for permanent accessibility to industry, academia and the public.  Before purchasing from NTIS, you may want to check for free access from (1) the issuing organization's website; (2) the U.S. Government Printing Office's Federal Digital System website http://www.gpo.gov/fdsys; (3) the federal government Internet portal USA.gov; or (4) a web search conducted using a commercial search engine such as http://www.google.com.
Accession Number ADA575880
Title Identification of Microbial Gene Biomarkers for in situ RDX Biodegradation.
Publication Date Dec 2012
Media Count 175p
Personal Author C. M. Jung D. E. Hancock F. H. Crocker K. J. Indest M. E. Merritt
Abstract Objectives of this project were to: (a) elucidate RDX degradation pathways in model RDX-degrading bacteria, (b) design and develop molecular tools to identify genes responsible for RDX biodegradation, and (c) correlate the response of biomarker(s) to concentrations of RDX and/or rates of RDX degradation. Gordonia sp. KTR9 and Shewanella oneidensis MR-1served as model bacterial systems for the aerobic and anaerobic degradation of RDX, respectively. Genome annotation and functional characterization of the plasmid pGKT2 in KTR9 revealed that xplA gene is both necessary and sufficient for RDX degradation. Shewanella oneidensis MR-1 was shown to efficiently degrade RDX anaerobically via two initial routes: (a) sequential N-NO2 reduction to the corresponding nitroso (N-NO) derivatives; and (b) mono-denitration followed by ring cleavage. The qPCR molecular tools described in this report have the potential to be used by remediation specialists for site characterization, treatment recommendations, and for evaluation and optimization of the treatment process. The fundamental information gained in this study suggests that XplA- mediated aerobic denitration of RDX may be subjected to inhibitory effects in response to nitrogen availability. Additional research is required to determine reliable guidelines to inform site managers of specific field concentrations of ammonium and nitrate that will increase RDX treatment times. Also, techniques to effectively lower the inorganic nitrogen concentrations to non-inhibitory levels for the aerobic RDX biodegradation pathway will need to be determined.
Keywords Assaying
Biodegradation
Carbon
Cytochrome oxidase
Cytosine
Deoxyribonucleic acids
Electron acceptors
Gene expression
Genes
Genome
Glnr(Global nitrogen transcription regulator protein)
Glutamine
Ground water
Guanosine
In situ analysis
Microbial gene biomarkers
Nitrogen
Proteins
Proteomics
Rdx
Soils
Strains(Biology)


 
Source Agency Non Paid ADAS
NTIS Subject Category 57B - Biochemistry
57F - Cytology, Genetics, & Molecular Biology
79A - Ammunition, Explosives, & Pyrotechnics
Corporate Author Army Engineer Waterways Experiment Station, Vicksburg, MS. Environmental Lab.
Document Type Technical report
Title Note Final rept.
NTIS Issue Number 1319
Contract Number N/A

Science and Technology Highlights

See a sampling of the latest scientific, technical and engineering information from NTIS in the NTIS Technical Reports Newsletter

Acrobat Reader Mobile    Acrobat Reader