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Accession Number ADA575599
Title Importance of Neurogenic Inflammation in Blast-Induced Neurotrauma.
Publication Date Jan 2013
Media Count 41p
Personal Author M. L. Schaefer
Abstract This project addresses the hypothesis that, in response to blast, blood-borne immune cells along with their secreted cytokines and chemokines from the periphery migrate via blood and infiltrate the CNS where they contribute to neuronal damage caused by activated microglia both in acute and chronic injury phases of blast-induced neurotrauma (BINT). To address the scientific feasibility of the hypothesis, we use state-of-the-art imaging and molecular techniques in mice with mild/moderate blast injury generated in a compressed helium-driven shock tube, throughout a one-month observation period. Progress & Results: At assigned time points after injury separate groups of animals with mild/moderate BINT are imaged by magnetic resonance imaging (MRI) to visualize potential macrophage infiltration; blood-brain barrier (BBB) disturbance; reactive gliosis; or astrocyte activation. The imaging findings were validated by immunocytochemistry. The obtained data suggest that a single exposure to mild/moderate blast induces both acute and chronic glial activation, levels of cytokines/chemokines, and motor impairment.
Keywords Bint(Blast induced neurotrauma)
Blast
Blood
Brain
Cells(Biology)
Central nervous system
Chemokines
Cytokines
Feasibility studies
Hypotheses
Images
Immune cells
Immunity
Infiltration(Fluids)
Inflammation
Magnetic resonance imaging
Motor disorders
Nerve cells
Nervous system
Shock tubes
Trauma
Traumatic brain injuries
Wounds and injuries


 
Source Agency Non Paid ADAS
NTIS Subject Category 57E - Clinical Medicine
57W - Stress Physiology
Corporate Author Johns Hopkins Univ., Baltimore, MD.
Document Type Technical report
Title Note Annual rept. 1 Jan-31 Dec 2012.
NTIS Issue Number 1319
Contract Number W81XWH-11-2-0071

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