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Accession Number ADA575222
Title Identification of Small Molecules Targeting the Posttranscriptional Control of ERG Expression.
Publication Date Oct 2012
Media Count 11p
Personal Author Y. Zhu
Abstract TMPRSS2-ERG translocation is involved in about 60% of prostate cancers. The translocation leads to overexpression of ERG which promotes cell proliferation and abrogates prostate epithelial differentiation. Suppression of ERG expression represents a major way to treat prostate cancers. However, there are no effective means to inhibit ERG expression. Although siRNA can deplete ERG expression, the big challenge is to deliver siRNA into prostate cancer cells. The mRNA stability, translation efficiency and protein stability of ERG are under strict control to maintain the ERG protein level. Here we report the establishment of a cell line which can be used to identify small molecules which inhibit ERG expression by targeting its post-transcriptional process. Using this cell line, we performed high-throughput screening and identified Gambogic acid which suppresses ERG expression in ERG-overexpressing prostate cancer cells.
Keywords Cells(Biology)
Deoxyribonucleic acids
Epithelium
Erg expression
High rate
Molecules
Post- transcriptional
Posttranscriptional control
Prostate cancer
Prostate carcinoma
Prostate gland
Proteins
Small molecules
Targeting
Translations


 
Source Agency Non Paid ADAS
NTIS Subject Category 57E - Clinical Medicine
Corporate Author Northwestern Univ., Evanston, IL.
Document Type Technical report
Title Note Final rept. 8 Mar 2011-7 Sep 2012.
NTIS Issue Number 1319
Contract Number W81XWH-11-1-0015

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