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Accession Number ADA570851
Title Glycoform of Immunoglobulin G (IgG) as an Early Biomarker of Exposure to Nonhuman Substances.
Publication Date Dec 2012
Media Count 34p
Personal Author A. S. Mehta S. Chen T. J. Henderson T. M. Block
Abstract We have identified an immunoglobulin G (IgG) subset that transiently exists in rabbits and mice following the introduction of various nonself antigens. This previously unrecognized IgG was named 'primebody' because it appears to be involved in priming the humoral immune response rather than in neutralizing immunogens. Primebody was initially identified by its ability to bind the Aleuria aurantia lectin (AAL) while still in its native conformation, in contrast to serum IgGs that bind AAL poorly, unless denatured. For both animals inoculated with three different immunogens, serum levels of primebody that were investigated with an AAL-antibody microarray assay reached peak levels 5 to 16 days post-inoculation. However, the immunogen-specific IgG levels peaked much later, between 21 to 60 days post-inoculation. Mass spectrometry analysis revealed 22 different primebody glycoisoforms in rabbits, all of which were under-galactosylated IgG (G0 and G1) with core fucose on its constant region N-glycan. We hypothesized that the immunoglobulin constant region heavy chain 2 (CH2) domain of primebody adopts an open conformation that allows AAL complete access to its glycans, in contrast with the usual closed conformation adopted by immunoglobulin CH2 domains, which are known to deny all glycan access.
Keywords Aal(Aleuria aurantia lectin)
Animals
Antigens
Arrays
Assaying
Biomarkers
Exposure(Physiology)
Glycoforms
Humoral immunity
Immunization
Immunoglobulins
Kinetics
Liquid chromatography
Mass spectrometry
Rabies virus
Receptor sites(Physiology)


 
Source Agency Non Paid ADAS
NTIS Subject Category 57B - Biochemistry
57S - Physiology
Corporate Author Army Research Development and Engineering Command, Aberdeen Proving Ground, MD.
Document Type Technical report
Title Note Final rept. Feb 2009-Feb 2012.
NTIS Issue Number 1315
Contract Number W911SR-09-C-0018

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