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Accession Number ADA570796
Title Truly Nonionic Polymer Shells for the Encapsulation of Living Cells.
Publication Date Jul 2011
Media Count 11p
Personal Author I. Drachuk J. L. Carter M. Stone N. Kelley-Loughnane S. Harbaugh
Abstract Engineering surfaces of living cells with natural or synthetic compounds can mediate intercellular communication and provide a protective barrier from hostile agents. We report on truly nonionic hydrogen-bonded LbL coatings for cell surface engineering. These ultrathin, highly permeable polymer membranes are constructed on living cells without the cationic component typically employed to increase the stability of LbL coatings. Without the cytotoxic cationic PEI prelayer, the viability of encapsulated cells drastically increases to 94%, in contrast to 20% viability in electrostatically-bonded LbL shells. Moreover, the long-term growth of encapsulated cells is not affected, thus facilitating efficient function of protected cells in a hostile environment.
Keywords Assaying
Atomic force microscopy
Barriers
Cell encapsulation
Cells(Biology)
Cytotoxins
Encapsulation
Fluorescence
Hydrogen bonds
Layers
Lbl(Layer-by-layer)
Macromolecules
Membranes
Microscopy
Morphology(Biology)
Polymers
Reprints
Roughness
Shells(Structural forms)
Tannic acid


 
Source Agency Non Paid ADAS
NTIS Subject Category 57F - Cytology, Genetics, & Molecular Biology
71 - Materials Sciences
Corporate Author Air Force Research Lab., Wright-Patterson AFB, OH. Human Effectiveness Directorate.
Document Type Journal article
Title Note Journal article.
NTIS Issue Number 1315
Contract Number FA9550-08-1-0446 FA9550-09-1-0162

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