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Accession Number ADA570551
Title Rapid Parallel Screening for Strain Optimization.
Publication Date Nov 2012
Media Count 5p
Personal Author C. Merryman T. Yee
Abstract Progress has been made on identifying biosensors that will be used to report on the fermentation yields of industrially relevant biological compounds. Screening of the desired chemicals has been largely completed. Microbes that can utilize these compounds as their sole carbon source have been isolated and archived. Phylotyping of the isolated microbes is nearly finished and genomic DNA has been prepared for sequencing. In this reporting period, we have completed the effort to screen the final 20 compounds from the initial set of chemicals. This set of 20 chemicals is more cumbersome than many of those already processed due to their physical properties (poor water solubility, etc). Most of these compounds were successfully used to isolate microbes that can utilize the chemicals as their sole carbon source. In total, almost 70 compounds have been paired with microbes as suggested by colony morphology. We have completed clonal purification, generated freezer stocks, phylotyped, and isolated genomic DNA for these. Growth screens for a final batch of 33 chemicals are underway and we expect to have these ready for sequencing in a couple of weeks. Except for the last batch of chemicals, phylotyping has been completed and we are currently analyzing the data. Genomic DNA from the isolated organisms has been prepared and will be submitted for sequencing on the Illumina HiSeq platform.
Keywords Biological compounds
Biological detection
Biomolecules
Biosensors
Carbon
Chemicals
Colony morphology
Deoxyribonucleic acids
Fermentation
Genome
Growth(General)
Isolation
Microbes
Microorganisms
Optimization
Orthogonality
Phylotyping
Physical properties
Platforms
Rapid parallel screening
Selection
Sequencing
Strains(Biology)
Synthetic biology

 
Source Agency Non Paid ADAS
NTIS Subject Category 57K - Microbiology
99D - Basic & Synthetic Chemistry
Corporate Author Defense Advanced Research Projects Agency, Arlington, VA.
Document Type Technical report
Title Note Quarterly rept. 17 Aug-16 Nov 2012.
NTIS Issue Number 1315
Contract Number HR0011-12-C-0062

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