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Accession Number ADA570546
Title Role of Mitochondrial Inheritance on Prostate Cancer Outcome in African American Men.
Publication Date Oct 2012
Media Count 10p
Personal Author M. Mims
Abstract We are examining the hypothesis that mitochondrial inheritance plays a significant role in aggressiveness of prostate cancer in African Americans. In the first year of the project we have identified 2,000 noncancerous tissues samples from African American men with prostate cancer and we have extracted DNA from approximately 1,500 of them to date. We have validated a robust new DNA sequencing technique developed by our collaborator using single amplicon long-range PCR that permits deep coverage (10,000-20,000X on average) of the mitochondrial genome. We have more than 300 samples in the sequencing pipeline with several hundred more to be submitted in the next few weeks. We have begun mapping of DNA variants in our sequenced genomes to mitochondrial genes. Illumina African Admixture mapping of the initial 72 subjects has been accomplished. Initial steps toward creating the Rho0 cell lines necessary for cybrid generation have begun with generation of puromycin resistant cell lines and testing of two different technologies for eliminating mitochondria from the nuclear donor cell lines.
Keywords African americans
Deoxyribonucleic acids
Genome
Mitochondria
Prostate cancer
Tissues(Biology)


 
Source Agency Non Paid ADAS
NTIS Subject Category 57E - Clinical Medicine
57F - Cytology, Genetics, & Molecular Biology
Corporate Author Baylor Coll. of Medicine, Houston, TX.
Document Type Technical report
Title Note Annual rept. 30 Sep 2011-29 Sep 2012.
NTIS Issue Number 1315
Contract Number W81XWH-11-1-0737

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