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Accession Number ADA570175
Title Detection of Non-hazardous, Fluorescent Ricin-B Via an Immunoassay on Simulated Plastic Wings.
Publication Date Sep 2012
Media Count 18p
Personal Author A. Hall G. Beatty
Abstract As a schedule 1 controlled substance, ricin is one of the deadliest plant toxins in existence, and also one of the most easily weaponizable. This combination has resulted in a recent surge of studies proposing new and improved methods of detecting ricin, primarily involving aqueous phase immunoassays. Successive methods report higher binding affinities and correspondingly lower detection limits, but little research has addressed the potential for new modes of implementation, extending beyond the aqueous phase. This study has examined the potential for detection along a solid/aerosol interface, increasing the variety of prospective scenarios to which the method can be applied. A solid plastic substrate treated with PolySorpTM, simulating a plastic wing surface, was used to anchor the potential antibody, a glycosphingolipid called monosialoganglioside (GM1). A monolayer of GM1 was introduced via direct adsorption followed by several rinsing steps, and verification of successful deposition is validated by Raman spectroscopy. The substrate-antibody complex is exposed to a known amount of fluorescently tagged, aerosolized ricin B (non-toxic analog), in a sealed, circulating environment.
Keywords Antibodies
Biological aerosols
Circulation
Exposure(General)
Fluorescence
Immunoassay
Limitations
Low level
Plants(Botany)
Plastics
Ricin
Scenarios
Scheduling
Substrates
Toxicity
Validation
Verification
Wings


 
Source Agency Non Paid ADAS
NTIS Subject Category 57B - Biochemistry
Corporate Author Army Research Lab., Aberdeen Proving Ground, MD. Vehicle Technology Directorate.
Document Type Technical report
Title Note Final rept. for period ending Jul 2012.
NTIS Issue Number 1314
Contract Number N/A

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