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Accession Number ADA570054
Title Validation of Biomarkers for Prostate Cancer Prognosis.
Publication Date Oct 2012
Media Count 10p
Personal Author J. D. Brooks Z. Feng
Abstract Our objective is to create a multi-institutional tissue microarray resource from radical prostatectomy samples with detailed clinical information and follow-up and rigorous casecohort design for use as a platform for validating tissue biomarkers of prognosis. In addition, we have proposed testing a series of biomarkers of prognosis and a set of biomarkers that correlate with Gleason Score. We have made significant progress over the past year. We have completed the tissue microarrays and finalized standard procedures for tissue microarray storage, sectioning and shipping. We have set up a structure for reviewing and approving biomarker proposals based on sound scientific principles and strong preliminary data. We have devised and tested a centralized distribution mechanism at Stanford University of collating and shipping TMAs to participating sites, We have found shortcomings with the BLISS system and STMAD for histological image capture and storage for pathological review and have developed a much improved, highly efficient system using a Leica scanner and PathXL image analysis software suite. We also have made significant progress in testing TACOMA, an automater TMA scoring algorithm. We have completed staining of the TMAs for H & E, High Molecular Weight Keratin, p27, ERG, SPKINK1, Ki67 (MIB1), MUC1, Survivin and PTEN FISH. Over the next year we will carry out more staining, complete refinements of the infrastructure, complete pathologic review of these and other biomarkers, evaluate concordance of scoring of immunohistochemical staining amongst study pathologists and report (publish) our findings.
Keywords Algorithms
Clinical medicine
Histology
Pathologists
Prostate cancer


 
Source Agency Non Paid ADAS
NTIS Subject Category 57A - Anatomy
57S - Physiology
57E - Clinical Medicine
Corporate Author Stanford Univ., CA.
Document Type Technical report
Title Note Annual rept. 30 Sep 2011-29 Sep 2012.
NTIS Issue Number 1314
Contract Number W81XWH-11-1-0380

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