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Accession Number ADA569754
Title Development of Technologies for Early Detection and Stratification of Breast Cancer.
Publication Date Oct 2012
Media Count 38p
Personal Author C. Kuperwasser D. Chiu D. R. Walt G. Sonenshein R. Buchsbaum
Abstract The overall goal of this work is to develop ultra-sensitive detection techniques to identify a panel of new biomarkers and indicators with diagnostic and predictive value in breast cancer. During year 1, we identified candidate breast cancer biomarkers and developed ultra-sensitive assays for several of them. Two different miRNA single molecule assay approaches have also been investigated and both showed promise for achieving similar ultrasensitive data using miRNA as targets. Continuing work on this assay includes increasing efficiency and decreasing the background. Circulating tumor cell isolation using microfluidics has been accomplished and these methods will soon be integrated with the single molecule detection approach. Good progress on identifying additional markers (miRNA, mtDNA, ADAM8) has been made. Further work to validate whether these markers are useful for detecting early stage breast cancer or tumor aggressiveness is underway. Finally, we have begun testing serum collected from a Human-in-Mouse (HIM) model of breast cancer using the assays developed.
Keywords Blood serum
Breast cancer
Cancer biomarkers
Circulating tumor cells
Early detection
Human-in-mouse model
Integrated systems
Mammary glands
Single cells
Single molecule detection
Ultra-sensitive protein assays

Source Agency Non Paid ADAS
NTIS Subject Category 57E - Clinical Medicine
Corporate Author Tufts Univ., Medford, MA.
Document Type Technical report
Title Note Annual rept. 29 Sep 2011-28 Sep 2012.
NTIS Issue Number 1314
Contract Number W81XWH-11-1-0814

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