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Accession Number
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ADA567663
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Title
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Molecular Regulation of Endothelial Cells by NF-1.
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Publication Date
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Jul 2012
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Media Count
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52p
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Personal Author
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K. Pumiglia
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Abstract
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We have a manuscript accepted that demonstrates the role of NF1in endothelial cell function. We have validated siRNA, antibodies, and inducible shRNA specific for individual Ras isoforms and used them to measure the regulation of Ras isoforms following loss of NF1 and their contributions to endothelial regulation, as well as the sufficiency of these isoforms to mimic the loss of NF1. As mTOR is critical to NF1 regulation, we investigated the role of this axis in endothelial cells. Lastly our attempts to create an in-vivo model of NF1 disease in the vasculature continue. We have succeeded in obtaining highly penetrant endothelial specific recombination. We have also bred this mouse onto the NF1 flox/flox background and evaluation of this phenotype is ongoing. We have in place a number of staining protocols and isolation procedures for biochemical analysis which should accelerate the determination of the effects in these mice. While this aspect of our proposed work has moved slower than anticipated, we are confident we now have the basis for an endothelial cell specific model of NF1.
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Keywords
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Blood vessels
Cells
Diseases
Endothelium
Functions
Molecules
Signaling
Vasculopathy
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Source Agency
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Non Paid ADAS
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NTIS Subject Category
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57A - Anatomy
57S - Physiology
57E - Clinical Medicine
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Corporate Author
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Albany Medical Coll., NY.
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Document Type
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Technical report
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Title Note
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Annual rept. 15 Jun 2011-14 Jun 2012.
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NTIS Issue Number
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1309
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Contract Number
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W81XWH-09-1-0432
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