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Accession Number
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ADA567337
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Title
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Reactivation of Breast Cancer Micrometastases by Senescent Bone Marrow Stroma.
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Publication Date
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Jul 2012
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Media Count
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23p
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Personal Author
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R. Wieder
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Abstract
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We developed an in vitro injury model of stroma cultured from bone marrow samples of mice and human donors. We demonstrated that oxidative and hypoxic damage and estrogen deprivation can induce IL-6 and IL-8 export by human female, premenopausal stroma. We adapted a breast cancer cell co-culture model to determine effect of injury on stromal capacity to support dormancy and demonstrated that breast cancer cells induce inflammatory cytokine secretion by stroma. We adapted an osteoclast activation model to determine the capacity of injured stroma to activate osteoclasts. We also adapted a preosteoblast co- culture model to determine the role of osteoblasts in the micrometastatic cell niche. Studies on estrogen replacement and anti-inflammatory treatment will determine reversibility.
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Keywords
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Bone marrow
Breast cancer
Cytokines
Donors(Medicine)
Estrogens
Tissues(Biology)
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Source Agency
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Non Paid ADAS
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NTIS Subject Category
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57A - Anatomy
57S - Physiology
57E - Clinical Medicine
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Corporate Author
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University of Medicine and Dentistry of New Jersey, Newark.
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Document Type
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Technical report
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Title Note
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Final rept. 1 Jul 2009-30 Jun 2012.
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NTIS Issue Number
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1309
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Contract Number
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W81XWH-09-1-0119
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