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Accession Number
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ADA566632
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Title
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B7-H4 as a Target for Breast Cancer Immunotherapy.
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Publication Date
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Jun 2012
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Media Count
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18p
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Personal Author
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A. L. Epstein J. K. Jang P. Hu
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Abstract
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B7-H4 is a recently discovered B7-family molecule that has been shown to inhibit T cell proliferation and secretion of IL-2. Therefore, it has been classified as an immunosuppressive protein. Protein expression has been limited to subsets of activated T cells and is inducible in dendritic cells and macrophages. In contrast, protein expression is abundant on tissues from several malignancies, most notably breast adenocarcinoma. We proposed to generate antagonistic humanized anti-B7- H4 antibodies for the reversal of immune escape generated in breast cancer. Here we report the generation of 64 mouse monoclonal antibodies for the detection of B7-H4 by ELISA, and 25 for the detection of cell surface B7-H4 by flow cytometry. We are currently assessing the 25 antibodies suitable for flow cytometry for direct cytotoxic effect on human breast cancer cell lines as well as for antagonistic effects on B7-H4 function. Candidate antibodies will be subsequently humanized using genetic engineering techniques. Here, we also report several novel findings not yet reported in published literature and not anticipated in our grant proposal.
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Keywords
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Antibodies
B7-h4 function
Blood counts
Breast cancer
Cells(Biology)
Cytotoxins
Detection
Documents
Escape systems
Immunosuppression
Immunotherapy
Mammary glands
Proteins
Secretion
T lymphocytes
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Source Agency
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Non Paid ADAS
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NTIS Subject Category
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57E - Clinical Medicine
57F - Cytology, Genetics, & Molecular Biology
57A - Anatomy
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Corporate Author
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University of Southern California, Los Angeles.
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Document Type
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Technical report
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Title Note
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Annual rept. 1 Jun 2011-31 May 2012.
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NTIS Issue Number
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1307
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Contract Number
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W81XWH-11-1-0466
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