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Accession Number
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ADA566631
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Title
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Snail-Induced Sulfonation Pathway in Breast Cancer Metastasis.
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Publication Date
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Jul 2012
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Media Count
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8p
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Personal Author
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I. F. Rauscher
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Abstract
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This projects seeks to establish a key role for cellular sulfonation enzymes in the metastatic progression of breast cancer. To this end we have accomplished the following tasks in this first year of support: 1. Creation Tetracycline inducible WT Snail and mutant Snail vectors for expression in cell lines, 2. Creation of stable cell lines in MCF10A cells and 67NR cells via a lentiviral expression system. 3. Demonstration MCF-10A cells undergo dramatic morphologic EMT in the presence of WT Snail expression, with the downregulation of the Cell adhesion molecule E-cadherin and the upregulation of the mesenchymal markers vimentin and fibronectin. 4. Characterization of antibodies to PAPSS1 and PAPSS2 enzymes. 5. Demonstration that both enzymes are expressed in Human breast cancer cell lines a with a positive correlation to Snail levels. 6. Derivation and characterization of shRNA knockdown vectors for PAPSS1 and PAPSS2.
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Keywords
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Antibodies
Breast cancer
Cells(Biology)
Cytochemistry
Demonstrations
Emt(Epithelial- mesenchymal transition)
Enzymes
Epithelium
Growth(Physiology)
Mcf10a cells
Metastasis
Molecules
Morphology
Mutations
Papss2 enzyme
Snails
Sulfonation
Tetracyclines
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Source Agency
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Non Paid ADAS
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NTIS Subject Category
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57Z - Zoology
57E - Clinical Medicine
48B - Natural Resource Management
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Corporate Author
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Wistar Inst. of Anatomy and Biology, Philadelphia, PA.
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Document Type
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Technical report
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Title Note
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Annual rept. 1 Jul 2011-30 Jun 2012.
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NTIS Issue Number
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1307
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Contract Number
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W81XWH-11-1-0494
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