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Accession Number ADA566501
Title Early Prediction of Lupus Nephritis Using Advanced Proteomics.
Publication Date Jun 2012
Media Count 65p
Personal Author P. Devarajan
Abstract The purpose of this project is to identify initial biomarker patterns in SLE nephritis using screening proteomic profiling. Utility of one of the biomarkers (NGAL) in predicting worsening of global and renal SLE disease activity has been validated. We have identified novel urinary biomarkers that distinguish between class IV and class V lupus nephritis, including albumin fragments and -1 acid glycoprotein(AGP) by 2D gel electrophoresis, transferrin (Tf), ceruloplasmin (Cp), lipocalin-type prostaglandin-D synthetase (L-PGDS), and a1-acid glycoprotein (AGP) by SELDI- TOF-MS, citrate, taurine and hippurate by NMR spectroscopy-based metabolomic profiling, and apolipoprotein D, lipocalin-like prostaglandin D synthetase, hemopexin, ceruloplasmin, -1 acid glycoprotein and orosomucoid by LCMS/MS profiling. We have validated whether these biomarkers are differentially expressed in patients with kidney biopsy-proven lupus nephritis types IV and V. Overall, these studies have identified non-invasive biomarkers that identify lupus nephritis sub-classes, and predict the clinical course of the disease. The significance of such biomarkers is that they will provide novel non- invasive tools to identify patients with lupus nephritis, to risk-stratify the subjects for therapies, and to follow the efficacy of therapies.
Keywords Albumins
Biomarkers
Kidneys
Lupus erythematosus
Lupus nephritis
Nephritis
Proteomics
Urine


 
Source Agency Non Paid ADAS
NTIS Subject Category 57A - Anatomy
57S - Physiology
57E - Clinical Medicine
Corporate Author Children's Hospital Medical Center, Cincinnati, OH.
Document Type Technical report
Title Note Final rept. 1 Jun 2007-31 May 2012.
NTIS Issue Number 1307
Contract Number W81XWH-07-1-0322

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