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Accession Number
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ADA565039
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Title
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Patient-Specific B-Cell Antibody Factories to Treat Metastatic Disease.
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Publication Date
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Aug 2012
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Media Count
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8p
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Personal Author
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K. Claffey
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Abstract
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Breast cancer patients often require surgical resection of tumor along with the tumor-draining lymph nodes which are termed sentinel lymph nodes. These sentinel lymph nodes are evaluated for any tumor cells which have metastasized from the primary tumor nodule, indicating higher grade tumors and informing clinical treatments for systemic chemotherapy. In this contract, we will apply novel technologies to identify highly reactive immune cells in patient-derived sentinel lymph nodes that indicate a patient response to unknown tumor antigenic peptides. In this initial report we have confirmed methods to identify the antibody producing B-cells from patient lymph nodes, isolated them from the tissue and cultured them in the laboratory. In addition, we have tested the tools needed to now immortalize these cultured cells and select those that react to that patients own tumor proteins/peptides. Active enrollment and processing of patient samples was delayed due to administrative approval delays, both internal and external, clinical interface training and education to assure optimal sample viability and lack of the unique immortalization virus, Epstein Barr Virus (EBV). All of these initial hurdles have been overcome and we are actively engaged in the proposed contract activities as indicated in our original proposal.
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Keywords
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Antibodies
Antigens
Breast cancer
Cells(Biology)
Chemotherapy
Clinical medicine
Epstein barr virus
Lymph nodes
Metastasis
Neoplasms
Peptides
Processing
Proteins
Reactivities
Response(Biology)
T lymphocytes
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Source Agency
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Non Paid ADAS
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NTIS Subject Category
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57E - Clinical Medicine
57J - Immunology
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Corporate Author
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Connecticut Univ. Health Center, Farmington.
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Document Type
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Technical report
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Title Note
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Annual rept. 1 Aug 2011-31 Jul 2012.
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NTIS Issue Number
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1304
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Contract Number
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W81XWH-11-1-0440
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