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Accession Number
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ADA564162
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Title
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Induced Pluripotent Stem Cells as Potential Therapeutic Agents in NF1.
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Publication Date
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May 2012
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Media Count
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9p
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Personal Author
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J. Chernoff
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Abstract
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In this project, we are seeking to use induced pluripotent stem (iPS) cell technology as a potential therapy in NF1. In the first years, we have successfully produced iPS cells from fobroblasts from Nf1 +/-mice and characterized the properties of these cells, which include growth in clusters, expression of stem cell markers, normal karyotype, and the ability to form teratomas in mice. We also created a targeting 'gene-repair' vector and have used this vector to to replace the defective Nf1 allele in these iPS cells. We then showed that we could differentiate iPS cells into Schwann-like cells in vitro, enabling drug sensitivity studies.
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Keywords
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Blood cells
Chemotherapeutic agents
Defects(Materials)
Differentiation
Drugs
Gene targeting
Growth(Physiology)
Hematopoietic cells
Ips(Induced pluriopotent stem)
Nf1(Neurofibromatosis 1)
Schwann cells
Stem cells
Vector analysis
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Source Agency
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Non Paid ADAS
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NTIS Subject Category
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57B - Biochemistry
57E - Clinical Medicine
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Corporate Author
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Institute for Cancer Research, Philadelphia, PA.
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Document Type
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Technical report
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Title Note
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Final rept. 1 May 2010-30 Apr 2010.
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NTIS Issue Number
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1302
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Contract Number
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W81XWH-10-1-0181
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