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Accession Number
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ADA564144
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Title
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Mining the Immune Cell Proteome to Identify Ovarian Cancer-Specific Biomarkers.
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Publication Date
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Mar 2012
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Media Count
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24p
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Personal Author
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M. S. Patankar
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Abstract
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In previous studies we have demonstrated that the ovarian cancer antigen CA125 specifically binds to certain sunsets of immune cell. Based on these observations we have hypothesized that proteomic and transciptomic analysis of immune cells from ovarian cancer patients will result in the identification of specific biomarkers in the immune cells. The current proposal will further investigate this hypothesis by conducting in-depth proteomic analysis of immune cells from cancer patients and healthy blood donors. Studies conducted have resulted in development of streamlined protocols for proteomic analysis of human immune cells. Proteomic analysis of human NK cells has been completed. In addition to proteomic analysis we have also compared the transcriptome of immune cells from ovarian cancer patients and healthy donors and have identified approximately 1600 genes that are differentially expressed in the patient samples. On-going research is focused on validating the proteomic and transcriptome data and demonstrating changes immune cells in response to cancer antigens.
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Keywords
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Antigens
Biomarker
Cells(Biology)
Detection
Early detection
Immune cells
Immunity
Microarray
Ovarian cancer
Proteomics
Transcriptome
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Source Agency
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Non Paid ADAS
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NTIS Subject Category
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57E - Clinical Medicine
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Corporate Author
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Wisconsin Univ.-Madison.
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Document Type
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Technical report
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Title Note
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Annual rept. 15 Feb 2011-14 Feb 2012.
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NTIS Issue Number
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1302
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Contract Number
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W81XWH-11-1-0181
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