Accession Number ADA563983
Title Interinstrument Variability and Validation Study for the XMX/2L-MIL Biological Air Sampler.
Publication Date Jul 2012
Media Count 13p
Personal Author C. W. Cooper J. E. Black
Abstract The interinstrument variability of the XMX/2L-MIL (XMX) biological air sampler was controlled by operating it with a fixed final nozzle orientation. Three XMXs were operated in a 12-m3 aerosol test chamber (ATC) in which a Bacillus globigii (Bg) aerosol was uniformly distributed for 18 experimental trials. An analysis of variance (ANOVA) was performed on the XMX results and had an Fvalue of 0.36, compared to the F-critical of 3.22 for a p- value of 0.05. This experiment was repeated using male-specific 2 bacteriophage (MS2) viral agent surrogate for 16 experimental trials. An ANOVA was performed on the XMX results and had an Fvalue of 0.09, compared to the F-critical of 3.22 for a p-value of 0.05. Therefore, it was concluded that operating XMXs at the specified final nozzle orientation would lead to precise laboratory culture results with acceptable interinstrument variability. Three XMXs were used to determine if Remel M5(registered trademark) collection media would better preserve MS2 for laboratory culture analysis than the commonly used PBS solution collection media. The XMXs were operated in the ATC in which MS2 aerosol was uniformly distributed for 16 experimental trials, with 8 conducted using Remel M5 and 8 with PBS solution as collection media. An ANOVA was performed on the XMX results and had an F-value of 6.73, compared to the F- critical of 5.30 for a p-value of 0.05. Therefore, it was concluded that Remel M5(registered trademark) produced superior results, as its culture results were 2.3 times greater than those for PBS solution.
Keywords Aerosols
Air sampling
Bacillus globigii
Bacillus subtilis
Bacteriophages
Biological aerosol
Biological agent
Biology
Collection media
Cultures(Biology)
Distribution
Laboratories
Laboratory tests
Liquid impinger
Nozzles
Remel(Registered trademark)
Samplers
Solutions(General)
Validation
Variations
Virtual impactor
Viruses


 
Source Agency Non Paid ADAS
NTIS Subject Category 99F - Physical & Theoretical Chemistry
41J - Tooling, Machinery, & Tools
Corporate Author Air Force School of Aerospace Medicine, Wright-Patterson AFB, OH.
Document Type Technical report
Title Note Letter rept. Mar 2011-May 2012.
NTIS Issue Number 1302
Contract Number N/A

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